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Single-Cell Assay innovation Develop Your Own Single-cell Multi-step Workflows

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Semi-Permeable Capsules (SPCs) are versatile microreactors that isolate cells and maintain their compartmentalization during bulk reactions. Their robustness and permeability enable seamless single-cell assay customization through simple buffer exchanges.

BULK–LIKE MULTI–STEP PROCESSING

In contrast to droplet-based systems, SPCs allow incompatible reactions (e.g. lysis and amplification) to be decoupled by removing previous reaction components through simple washing, ensuring each reaction occurs under optimal conditions.

This flexibility empowers a mix-and-match approach to profile the single-cell genome, transcriptome, epigenome, and beyond.

1. Prepare

Lyse
  • pH 2.0-13.0
  • Harsh lysis reagents (SDS, GITC, alkaline)
  • High temperature (up to 100°C)
Fix
  • Methanol
  • Ethanol
  • Aldehyde fixatives
Purify
  • Proteinase K
  • RNases
  • DNases
Cultivate
Nutrients exchange freely sustaining cell growth

2. Process

AMPLIFY
  • Isothermal amplification
  • PCR
  • Reverse transcription
Barcode
  • Nucleic acid barcoding
MODIFY
  • DNA fragmentation
  • End repair
  • Ligation
STAIN
  • Fluorescent dyes
  • Antibodies
  • FISH probes
RETAIN
Capsules sealed in oil prevent small molecule leakage
CUSTOM
No reagent lock-in

3. Finalize

SEQUENCE
  • Short–read Platforms
  • Long–read Platforms
DETECT/SELECT
  • Microscopy
  • FACS
RELEASE
Dissolve SPCs at room temperature without impacting cells
Store
  • +4°C (aqueous)
  • -80°C (cryopreservants)
White paper: The overview of the Semi-Permeable Capsule (SPC) technology
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Publications featuring Single Cell Assay Innovation

Systematic benchmarking of single-cell ATAC-sequencing protocols
Read publication
High throughput Single Cell Metagenomic Sequencing with Semi-Permeable Capsule: Unraveling Microbial Diversity at the Single-Cell Level in Sewage and Feces Microbiomes
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RNA cytometry of single-cells using semi-permeable microcapsules
Read publication
Single-cell barcoding and sequencing using droplet microfluidics
Read publication
Hydrop enables droplet-based single-cell ATAC-seq and single-cell RNA-seq using dissolvable hydrogel beads
Read publication
inDrops-2: a flexible, versatile and cost-efficient droplet microfluidic approach for high-throughput scRNA-seq of fresh and preserved clinical samples
Read publication

ENRICHMENT

SPCs are compatible with standard FACS instrumentation, enabling the enrichment of fluorescent populations of interest. Capsules can also be sealed by reemulsifying them in oil, preventing small molecule leakage and enabling seamless sorting on the Styx platform.

FACS
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Read Technical Note

GENERATE SPCs and INNOVATE YOUR WAY

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EXCITED TO ENABLE

Sequencing

Single-cell DNA-seq
Targeted sequencing
Single-cell multiomic sequencing
Immune profiling
Single-cell accessible chromatin profiling

MICROBIOLOGY

Antimicrobial resistance surveillance
Cultivation of anaerobic microorganisms
Extremophiles research
Viral genomics

OTHER

Spheroid formation
Nucleic acid cytometry
Co-culturing

Start with SEMI–PERMEABLE CAPSULE GENERATION

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